LC-LCMS Best Practices in Application Method Development/Operation and Maintenance

An overview of LC –MS applications, including food, environmental, industrial, GPC, and biopharmaceutical analysis

• Concepts, perspectives, best practices, and potential issues surrounding UHPLC • Fundamentals of LC-MS method development and easier approaches • Overview of method validation and transfer • Standard operating procedures for LC-MS modules, and troubleshooting strategie  • Introductionto LC-MS and Principles of LC-MS                                                            • Theory of Single Quadrupole, Triple Quadrupole, System overview Ionization sources, detectors, Collision induced Dissociation, Solvents, buffers & additives used in LC-MS • MS operation, including the operation of the most popular LC MS interfaces • Operation in MS, MS/MS, and MS/MS/MS modes  Tuning and Calibration, Product Ion Scan, MRM Method and  HPLC Method  • Ion production, fragmentation, and detection • MS calibration and optimization. • Set up method for Full Scan and Single Ion Monitoring operation   LC-MS Sample Preparation for Pesticide Analysis • Sample Preparation Techniques • SPE, solvent extraction • Quenchers Method, MSPD: Matrix Solid Phase Dispersion                                          LC –MS Sample Preparation for analysis of Organic pollutants 

• Sample Preparation Techniques for Drug residue analysis • SPE, solvent extraction  • Quenchers Method  • MSPD: Matrix Solid Phase Dispersion                                                                          • Creating sequence for multiple sample analysis of pesticide residue • Developing calibration curve.  • Method optimizations and the validations of parameters such as LOD, LOQ, accuracy, precision, linearity and robustness in LC-MS-MS • Sample analysis of Pesticide residue in Water samples, Quantitative analysis of Organic pollutants

• Quantitative data analysis with set files • Quantitation using internal standard

Maintenance and Troubleshooting – Effectively  detecting, troubleshooting and rectifying common  issues – Performing instrument maintenance  Carrying out relevant diagnostic tests – Experience  from hands-on laboratory exercises