HPLC/UHPLC BEST PRACTICES IN METHOD DEVELOPMENT/OPEARTIONS AND TROUBLE SHOOTING

Chrom Africa offers an outstanding platform for training on HPLC. This  training program provides theoretical and practical understanding of HPLC,  its maintenance, method development and troubleshooting techniques. All  the information related to latest available HPLC components from pumps and  detectors is provided. During the training, knowledge of selecting relevant  instrumentation and columns, Detectors, Sample preparations, creating  methods interpreting and troubleshooting the chromatograms is imparted.  Hands-on aspect to enhance the practical operational experience is provided.

 • Introduction & Instrumentation of HPLC, functions and  operation HPLC components

 • Introduction to basic principle of chromatography, various  HPLC detectors with their uses, columns, binary and isocratic  gradients, mobile phases, polarity and retention time

 • Functioning of UV/VIS/DAD/RID detectors, its working,  calibration and applications. • Functioning of, Fluorescence detector functioning, its working,  calibration and applications and other HPLC Detectors.

 • HPLC Columns selections and types of Columns. • HPLC Columns installation procedure and care of columns

 • Creating method on LC Software and validating the method.  (Practical) • Development of method, optimizing various chromatographic  parameters

 • LC Sample Preparation and clean up ( Practical’s)

 • Preparation of test solutions, Mobile Phase, Setup method for  HPLC, Data acquisition software. (Practical)

• Creating sequence and multiple sample analysis (Practical) • Developing calibration curve and producing report files.  (Practical)

 • Sample Analysis ( Practical’s)

 • Quantitation software, Integration and Report Generation

  • Analysis of different types of water samples

 • Quantitation using internal standards • Understanding specific applications, preventive  care, maintenance and trouble shooting.  Practical)